ABI1 ELISA Kit

Catalog No. ABIN5651333

Product Details ABI1 ELISA Kit

Target: ABI1 (Abl-Interactor 1 (ABI1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Abl Interactor 1 (ABI1). No significant cross-reactivity or interference between Abl Interactor 1 (ABI1) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Abl Interactor 1 (ABI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Abl Interactor 1 (ABI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Abl Interactor 1 (ABI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Abl Interactor 1 (ABI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Abl Interactor 1 (ABI1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Abl Interactor 1 (ABI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ABI1

Target: ABI1 (Abl-Interactor 1 (ABI1))

Alternative Name: Abl Interactor 1 (ABI1 Products)

Synonyms: ABI-1 ELISA Kit, ABLBP4 ELISA Kit, E3B1 ELISA Kit, NAP1BP ELISA Kit, SSH3BP ELISA Kit, SSH3BP1 ELISA Kit, NAP1 ELISA Kit, Ssh3bp1 ELISA Kit, E3b1 ELISA Kit, abi1 ELISA Kit, wu:fc66g09 ELISA Kit, wu:fk56a10 ELISA Kit, zgc:73174 ELISA Kit, MGC84356 ELISA Kit, ABI1 ELISA Kit, abi-1 ELISA Kit, e3b1 ELISA Kit, nap1bp ELISA Kit, ssh3bp ELISA Kit, ssh3bp1 ELISA Kit, wu:fe48a10 ELISA Kit, zgc:153534 ELISA Kit, DKFZp469P1319 ELISA Kit, abl interactor 1 ELISA Kit, abl-interactor 1 ELISA Kit, abl-interactor 1a ELISA Kit, abl interactor 1 S homeolog ELISA Kit, abl-interactor 1b ELISA Kit, Abl-interactor 1 ELISA Kit, Abl interactor 1 ELISA Kit, ABI1 ELISA Kit, Abi1 ELISA Kit, abi1a ELISA Kit, abi1.S ELISA Kit, abi1 ELISA Kit, abi1b ELISA Kit, Bm1_44660 ELISA Kit, LOAG_04356 ELISA Kit

Background:

Gene Name: Abl Interactor 1

Gene Aliases: ABI-1, E3B1, NAP1BP, SSH3BP1, Spectrin SH3 Domain Binding Protein 1, Abelson interactor 1, Abl-binding protein 4, Nap1-binding protein, Eps8 SH3 domain-binding protein

Gene ID: 10006

UniProt: Q8IZP0

Pathways: RTK Signaling, Response to Water Deprivation, ER-Nucleus Signaling