ADAM28 ELISA Kit

Catalog No. ABIN5651313

Product Details ADAM28 ELISA Kit

Target: ADAM28 (ADAM Metallopeptidase Domain 28 (ADAM28))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 28 (ADAM28). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 28 (ADAM28) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 28 (ADAM28). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 28 (ADAM28). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 28 (ADAM28), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 28 (ADAM28) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 28 (ADAM28) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 28 (ADAM28) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ADAM28

Target: ADAM28 (ADAM Metallopeptidase Domain 28 (ADAM28))

Alternative Name: A Disintegrin And Metalloprotease 28 (ADAM28 Products)

Synonyms: C130072N01Rik ELISA Kit, D430033C21Rik ELISA Kit, Dtgn1 ELISA Kit, MDC-L ELISA Kit, MDC-Lm ELISA Kit, MDC-Ls ELISA Kit, MDCL ELISA Kit, TECADAM ELISA Kit, eMDCII ELISA Kit, ADAM 28 ELISA Kit, ADAM23 ELISA Kit, eMDC II ELISA Kit, ADAM metallopeptidase domain 28 ELISA Kit, a disintegrin and metallopeptidase domain 28 ELISA Kit, Adam28 ELISA Kit, ADAM28 ELISA Kit

Background:

Gene Name: A Disintegrin And Metalloprotease 28

Gene Aliases: ADAM23, MDCL, eMDCII, Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L, Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II

Gene ID: 10863

UniProt: Q9UKQ2