ADAM17 ELISA Kit

Catalog No. ABIN5651310

Product Details ADAM17 ELISA Kit

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 17 (ADAM17). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 17 (ADAM17) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 17 (ADAM17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 17 (ADAM17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 17 (ADAM17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 17 (ADAM17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 17 (ADAM17) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 17 (ADAM17) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ADAM17

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: A Disintegrin And Metalloprotease 17 (ADAM17 Products)

Synonyms: ADAM18 ELISA Kit, CD156B ELISA Kit, CSVP ELISA Kit, NISBD ELISA Kit, TACE ELISA Kit, CD156b ELISA Kit, Tace ELISA Kit, adam17 ELISA Kit, si:dkey-81b15.2 ELISA Kit, adam18 ELISA Kit, cd156b ELISA Kit, csvp ELISA Kit, tace ELISA Kit, fc20b01 ELISA Kit, wu:fc20b01 ELISA Kit, zgc:63886 ELISA Kit, ADAM metallopeptidase domain 17 ELISA Kit, a disintegrin and metallopeptidase domain 17 ELISA Kit, ADAM metallopeptidase domain 17b ELISA Kit, ADAM metallopeptidase domain 17 L homeolog ELISA Kit, ADAM metallopeptidase domain 17a ELISA Kit, ADAM17 ELISA Kit, Adam17 ELISA Kit, adam17b ELISA Kit, adam17.L ELISA Kit, adam17a ELISA Kit

Background:

Gene Name: A Disintegrin And Metalloprotease 17

Gene Aliases: CD156b, TACE, CSVP, Tumor Necrosis Factor,Alpha,Converting Enzyme, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease

Gene ID: 57027

UniProt: Q9Z1K9

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus