Serotonin Receptor 1A ELISA Kit

Catalog No. ABIN5651286

Product Details Serotonin Receptor 1A ELISA Kit

Target: Serotonin Receptor 1A (HTR1A)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of 5-Hydroxytryptamine Receptor 1A (HTR1A). No significant cross-reactivity or interference between 5-Hydroxytryptamine Receptor 1A (HTR1A) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxytryptamine Receptor 1A (HTR1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5-Hydroxytryptamine Receptor 1A (HTR1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Hydroxytryptamine Receptor 1A (HTR1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine Receptor 1A (HTR1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxytryptamine Receptor 1A (HTR1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxytryptamine Receptor 1A (HTR1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Serotonin Receptor 1A

Target: Serotonin Receptor 1A (HTR1A)

Alternative Name: 5-Hydroxytryptamine Receptor 1A (HTR1A Products)

Synonyms: 5-HT-dro2A ELISA Kit, 5-HT1ADro ELISA Kit, 5-HT[1A]Dro ELISA Kit, 5-HT[[1ADro]] ELISA Kit, 5-HT[[1A]] ELISA Kit, 5-HT[[1A]]DRO ELISA Kit, 5HT-R2A ELISA Kit, 5HT-dro2A ELISA Kit, 5HT-drp[[2A]] ELISA Kit, 5HT1A ELISA Kit, 5HT[[1ADro]] ELISA Kit, 5htdro2a ELISA Kit, CG16720 ELISA Kit, DRO2A ELISA Kit, Dm5HTdro2A ELISA Kit, Dmel\\CG16720 ELISA Kit, d5-HT1A ELISA Kit, 5htr1a ELISA Kit, htr1a ELISA Kit, HTR1A ELISA Kit, Htr1a ELISA Kit, 5-Ht1a ELISA Kit, Gpcr18 ELISA Kit, 5-HT-1A ELISA Kit, 5-HT1A ELISA Kit, 5HT1a ELISA Kit, ADRB2RL1 ELISA Kit, ADRBRL1 ELISA Kit, G-21 ELISA Kit, PFMCD ELISA Kit, RAT5HT1A ELISA Kit, 5ht1a ELISA Kit, adrb2rl1 ELISA Kit, adrbrl1 ELISA Kit, htr1a-A ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 1A ELISA Kit, 5-hydroxytryptamine receptor 1A ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 1A a ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled L homeolog ELISA Kit, 5-HT1A ELISA Kit, HTR1A ELISA Kit, htr1aa ELISA Kit, Htr1a ELISA Kit, htr1a.L ELISA Kit

Background:

Gene Name: 5-Hydroxytryptamine Receptor 1A

Gene Aliases: 5HTR1A, 5HTR1-A, 5-HTR1-A, ADRB2RL1, ADRBRL1, Serotonin Receptor 1A

Pathways: JAK-STAT Signaling, Synaptic Membrane, Feeding Behaviour