5-HETE ELISA Kit

Catalog No. ABIN5651283

Product Details 5-HETE ELISA Kit

Target: 5-HETE (5-Hydroxyeicosatetraenoic Acid (5-HETE))

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 9.88 ng/mL - 800 ng/mL

Minimum Detection Limit: 9.88 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of 5-Hydroxyeicosatetraenoic Acid (5-HETE). No significant cross-reactivity or interference between 5-Hydroxyeicosatetraenoic Acid (5-HETE) and analogues was observed.

Sensitivity: 3.95 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Hydroxyeicosatetraenoic Acid (5-HETE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxyeicosatetraenoic Acid (5-HETE) and unlabeled 5-Hydroxyeicosatetraenoic Acid (5-HETE) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxyeicosatetraenoic Acid (5-HETE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxyeicosatetraenoic Acid (5-HETE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Hydroxyeicosatetraenoic Acid (5-HETE) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxyeicosatetraenoic Acid (5-HETE) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxyeicosatetraenoic Acid (5-HETE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for 5-HETE

Target: 5-HETE (5-Hydroxyeicosatetraenoic Acid (5-HETE))

Alternative Name: 5-Hydroxyeicosatetraenoic Acid (5-HETE Products)

Background:

Gene Name: 5-Hydroxyeicosatetraenoic Acid