HSD17B10 ELISA Kit

Catalog No. ABIN5651261

Product Details HSD17B10 ELISA Kit

Target: HSD17B10 (Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10). No significant cross-reactivity or interference between 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) and analogues was observed.

Sensitivity: 0.063 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HSD17B10

Target: HSD17B10 (Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10))

Alternative Name: 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17B10 Products)

Synonyms: hadh2 ELISA Kit, im:6899159 ELISA Kit, wu:fj61f03 ELISA Kit, zgc:101605 ELISA Kit, abad ELISA Kit, camr ELISA Kit, erab ELISA Kit, hcd2 ELISA Kit, hsd10 ELISA Kit, mhbd ELISA Kit, mrpp2 ELISA Kit, mrx17 ELISA Kit, mrx31 ELISA Kit, mrxs10 ELISA Kit, schad ELISA Kit, sdr5c1 ELISA Kit, HADH2 ELISA Kit, HSD17B10 ELISA Kit, 17b-HSD10 ELISA Kit, ABAD ELISA Kit, CAMR ELISA Kit, DUPXp11.22 ELISA Kit, ERAB ELISA Kit, HCD2 ELISA Kit, MHBD ELISA Kit, MRPP2 ELISA Kit, MRX17 ELISA Kit, MRX31 ELISA Kit, MRXS10 ELISA Kit, SCHAD ELISA Kit, SDR5C1 ELISA Kit, 17bHSD10 ELISA Kit, Ads9 ELISA Kit, Hadh2 ELISA Kit, hydroxysteroid (17-beta) dehydrogenase 10 ELISA Kit, hydroxysteroid 17-beta dehydrogenase 10 ELISA Kit, hydroxysteroid (17-beta) dehydrogenase 10 L homeolog ELISA Kit, HSD17B10 ELISA Kit, hsd17b10 ELISA Kit, hsd17b10.L ELISA Kit, Hsd17b10 ELISA Kit

Background:

Gene Name: 17-Beta-Hydroxysteroid Dehydrogenase Type 10

Gene Aliases: 17b-HSD10, ABAD, ERAB, HADH2, HCD2, MHBD, SCHAD, SDR5C1, MRPP2, CAMR, 3-Hydroxyacyl-CoA Dehydrogenase Type-2, AB-Binding Alcohol Dehydrogenase

UniProt: O08756