NAPA ELISA Kit
NAPA
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Cells, Plasma, Serum
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- Target See all NAPA ELISA Kits
- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Brand
- AssayMax™
- Sample Type
- Cell Culture Cells, Plasma, Serum
- Analytical Method
- Quantitative
- Material not included
- Microplate reader capable of measuring absorbance at 405 nm. Pipettes (1-20 µL, 20-200 µL, and multiple channel). Deionized or distilled reagent grade water Incubator (37 °C)
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- Assay Time
- 4 h
- Plate
- Pre-coated
- Calculation of Results
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- Calculate the mean value of the duplicate or triplicate readings for each standard and sample.
- To generate a standard curve, plot the graph using the standard concentrations on the x-axis and the corresponding mean 450 nm absorbance (OD) on the y-axis. The best-fit line can be determined by regression analysis using log-log or four-parameter logistic curve-fit.
- Determine the unknown sample concentration from the standard curve and multiply the value by the dilution factor.
- Restrictions
- For Research Use only
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- Handling Advice
- This product is for Research Use Only and is Not For Use In Diagnostic Procedures. Prepare all reagents (working diluent buffer, wash buffer, standard, biotinylated antibody, and SP Conjugate) as instructed, prior to running the assay. Prepare all samples prior to running the assay. The dilution factors for the samples are suggested in this insert. However, the user should determine the optimal dilution factor. Spin down the SP Conjugate vial and the biotinylated antibody vial before opening and using contents. The Stop Solution is an acidic solution. The kit should not be used beyond the expiration date. 2
- Storage
- 4 °C,-20 °C
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- Target See all NAPA ELISA Kits
- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
- Alternative Name
- SNAP-alpha (NAPA Products)
- Synonyms
- SNAPA ELISA Kit, wu:fa26c04 ELISA Kit, zgc:63906 ELISA Kit, napb ELISA Kit, 1500039N14Rik ELISA Kit, AW209189 ELISA Kit, RA81 ELISA Kit, SNARE ELISA Kit, a-SNAP ELISA Kit, hyh ELISA Kit, alpha-SNAP ELISA Kit, Alpha-SNAP ELISA Kit, NSF attachment protein alpha ELISA Kit, N-ethylmaleimide-sensitive factor attachment protein, alpha b ELISA Kit, NSF attachment protein alpha L homeolog ELISA Kit, N-ethylmaleimide-sensitive factor attachment protein, alpha ELISA Kit, N-ethylmaleimide sensitive fusion protein attachment protein alpha ELISA Kit, soluble NSF attachment protein ELISA Kit, NAPA ELISA Kit, napab ELISA Kit, napa.L ELISA Kit, napa ELISA Kit, EHI_000300 ELISA Kit, Napa ELISA Kit, LOC102577508 ELISA Kit
- Gene ID
- 8775
- UniProt
- P54920
- Pathways
- Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
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