LDHB ELISA Kit

Catalog No. ABIN5564579

Product Details LDHB ELISA Kit

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Brand: AssayMax™

Sample Type: Cell Culture Cells, Plasma, Serum

Analytical Method: Quantitative

Material not included: Microplate reader capable of measuring absorbance at 405 nm. Pipettes (1-20 µL, 20-200 µL, and multiple channel). Deionized or distilled reagent grade water Incubator (37 °C)

Application Details

Assay Time: 4 h

Plate: Pre-coated

Calculation of Results:

• Calculate the mean value of the duplicate or triplicate readings for each standard and sample.
• To generate a standard curve, plot the graph using the standard concentrations on the x-axis and the corresponding mean 450 nm absorbance (OD) on the y-axis. The best-fit line can be determined by regression analysis using log-log or four-parameter logistic curve-fit.
• Determine the unknown sample concentration from the standard curve and multiply the value by the dilution factor.

Restrictions: For Research Use only

Handling

Handling Advice: This product is for Research Use Only and is Not For Use In Diagnostic Procedures. Prepare all reagents (working diluent buffer, wash buffer, standard, biotinylated antibody, and SP Conjugate) as instructed, prior to running the assay. Prepare all samples prior to running the assay. The dilution factors for the samples are suggested in this insert. However, the user should determine the optimal dilution factor. Spin down the SP Conjugate vial and the biotinylated antibody vial before opening and using contents. The Stop Solution is an acidic solution. The kit should not be used beyond the expiration date. 2

Storage: 4 °C,-20 °C

Target Details for LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Alternative Name: L-Lactate Dehydrogenase B Chain (LDH-B) (LDHB Products)

Synonyms: LDH-B ELISA Kit, LDH-H ELISA Kit, LDHBD ELISA Kit, TRG-5 ELISA Kit, AI790582 ELISA Kit, H-Ldh ELISA Kit, Ldh-2 ELISA Kit, Ldh2 ELISA Kit, ldh-h ELISA Kit, ldh2 ELISA Kit, ldhb ELISA Kit, ldhba ELISA Kit, trg-5 ELISA Kit, LDHB ELISA Kit, LDH-B-A ELISA Kit, wu:fa16d07 ELISA Kit, wu:fa20c10 ELISA Kit, wu:fa96a08 ELISA Kit, LDH-B-B ELISA Kit, zgc:92882 ELISA Kit, lactate dehydrogenase B ELISA Kit, lactate dehydrogenase B S homeolog ELISA Kit, lactate dehydrogenase Ba ELISA Kit, lactate dehydrogenase Bb ELISA Kit, LDHB ELISA Kit, Ldhb ELISA Kit, ldhb.S ELISA Kit, ldhb ELISA Kit, ldhba ELISA Kit, ldhbb ELISA Kit

Gene ID: 3945

UniProt: P07195

Pathways: Warburg Effect