S100A16 ELISA Kit
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- Target See all S100A16 ELISA Kits
- S100A16 (S100 Calcium Binding Protein A16 (S100A16))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Brand
- AssayMax™
- Sample Type
- Cell Culture Cells, Plasma, Serum
- Analytical Method
- Quantitative
- Material not included
- Microplate reader capable of measuring absorbance at 405 nm. Pipettes (1-20 µL, 20-200 µL, and multiple channel). Deionized or distilled reagent grade water Incubator (37 °C)
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S100 Calcium Binding Protein A16 (S100A16) ELISA Kit
S100A16 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A16 (S100A16) ELISA KitS100A16 Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A16 (S100A16) ELISA KitS100A16 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- Plate
- Pre-coated
- Calculation of Results
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- Calculate the mean value of the duplicate or triplicate readings for each standard and sample.
- To generate a standard curve, plot the graph using the standard concentrations on the x-axis and the corresponding mean 450 nm absorbance (OD) on the y-axis. The best-fit line can be determined by regression analysis using log-log or four-parameter logistic curve-fit.
- Determine the unknown sample concentration from the standard curve and multiply the value by the dilution factor.
- Restrictions
- For Research Use only
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- Handling Advice
- This product is for Research Use Only and is Not For Use In Diagnostic Procedures. Prepare all reagents (working diluent buffer, wash buffer, standard, biotinylated antibody, and SP Conjugate) as instructed, prior to running the assay. Prepare all samples prior to running the assay. The dilution factors for the samples are suggested in this insert. However, the user should determine the optimal dilution factor. Spin down the SP Conjugate vial and the biotinylated antibody vial before opening and using contents. The Stop Solution is an acidic solution. The kit should not be used beyond the expiration date. 2
- Storage
- 4 °C,-20 °C
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- Target See all S100A16 ELISA Kits
- S100A16 (S100 Calcium Binding Protein A16 (S100A16))
- Alternative Name
- S100-A16 (S100A16 Products)
- Synonyms
- DT1P1A7 ELISA Kit, S100F ELISA Kit, 2300002L21Rik ELISA Kit, AI325039 ELISA Kit, AI663996 ELISA Kit, S100 calcium binding protein A16 ELISA Kit, S100A16 ELISA Kit, S100a16 ELISA Kit
- Gene ID
- 140576
- UniProt
- Q96FQ6
- Pathways
- S100 Proteins
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