CHEK1 ELISA Kit

Catalog No. ABIN5526744

Product Details CHEK1 ELISA Kit

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Binding Specificity: pSer280, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Purpose: Human and Mouse Phospho-Rictor (Thr1135) and Total Rictor Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures CHK1 phosphorylated at Serine-280 as well as total CHK1 in adherent cell lines.

Sample Type: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes Human CHK1 phosphorylated at site Serine-280 as well as total CHK1.

Characteristics:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Components:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1 (CHEK1 Products)

Synonyms: An08g10320 ELISA Kit, AO090003000441 ELISA Kit, CHK1 ELISA Kit, chk1 ELISA Kit, C85740 ELISA Kit, Chk1 ELISA Kit, rad27 ELISA Kit, id:ibd2720 ELISA Kit, zgc:56093 ELISA Kit, CHEK1 ELISA Kit, checkpoint kinase 1 ELISA Kit, serine/threonine-protein kinase chk1 ELISA Kit, serine/threonine-protein kinase Chk1 ELISA Kit, CAMK/CAMKL/CHK1 protein kinase Chk1 ELISA Kit, checkpoint kinase 1 S homeolog ELISA Kit, CHEK1 ELISA Kit, ANI_1_2436074 ELISA Kit, AOR_1_768154 ELISA Kit, PTRG_04183 ELISA Kit, SJAG_01680 ELISA Kit, PAAG_04978 ELISA Kit, MCYG_03290 ELISA Kit, VDBG_03742 ELISA Kit, chek1.S ELISA Kit, Chek1 ELISA Kit, chek1 ELISA Kit

Gene ID: 1111

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair