Caveolin-1 ELISA Kit

Catalog No. ABIN5526738

Product Details Caveolin-1 ELISA Kit

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Binding Specificity: pTyr14, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Purpose: Human Phospho-NBS1 (Ser343) and Total NBS1 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures Caveolin-1 phosphorylated at Tyrosine-14 as well as total Caveolin-1 in adherent cell lines.

Sample Type: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes Human Caveolin-1 phosphorylated at site Tyrosine-14 as well as total Caveolin-1.

Characteristics:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Components:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: Caveolin-1 (CAV1 Products)

Synonyms: fc07c04 ELISA Kit, wu:fc07c04 ELISA Kit, Cav-1 ELISA Kit, bscl3 ELISA Kit, cav1 ELISA Kit, cav1a ELISA Kit, cgl3 ELISA Kit, vip21 ELISA Kit, cav1b ELISA Kit, caveolin-1 ELISA Kit, cav ELISA Kit, mstp085 ELISA Kit, Caveolin-1 ELISA Kit, CAV1 ELISA Kit, cav-1 ELISA Kit, Cav ELISA Kit, caveolin 1 ELISA Kit, BSCL3 ELISA Kit, CGL3 ELISA Kit, MSTP085 ELISA Kit, PPH3 ELISA Kit, VIP21 ELISA Kit, caveolin 1 ELISA Kit, caveolin 1 L homeolog ELISA Kit, caveolin 1 S homeolog ELISA Kit, caveolin 1, caveolae protein ELISA Kit, caveolin 1, caveolae protein, 22kDa ELISA Kit, Caveolin ELISA Kit, cav1 ELISA Kit, cav1.L ELISA Kit, cav1.S ELISA Kit, CAV1 ELISA Kit, Cav1 ELISA Kit, cav-1 ELISA Kit

Gene ID: 857

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals