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PAK2 ELISA Kit

PAK2 Reactivity: Human pSer20 Colorimetric Cell ELISA Adherent Cell Culture
Catalog No. ABIN5526726
  • Target See all PAK2 ELISA Kits
    PAK2 (P21-Activated Kinase 2 (PAK2))
    Binding Specificity
    pSer20, total
    Reactivity
    • 7
    • 4
    • 4
    Human
    Detection Method
    Colorimetric
    Method Type
    Cell ELISA
    Application
    ELISA
    Purpose
    Cell-Based ELISA Kit. This assay semi-quantitatively measures PAK2 phosphorylated at Serine-20 as well as total PAK2 in adherent cell lines.
    Sample Type
    Adherent Cell Culture
    Analytical Method
    Semi-Quantitative
    Specificity
    This ELISA kit recognizes Human PAK2 phosphorylated at site Serine-20 as well as total PAK2.
    Characteristics
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Components
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product PAK2 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all PAK2 ELISA Kits
    PAK2 (P21-Activated Kinase 2 (PAK2))
    Alternative Name
    PAK2 (PAK2 Products)
    Synonyms
    PAK65 ELISA Kit, PAKgamma ELISA Kit, gamma-PAK ELISA Kit, 5330420P17Rik ELISA Kit, A130002K10Rik ELISA Kit, AI836325 ELISA Kit, D16Ertd269e ELISA Kit, PAK-2 ELISA Kit, mKIAA4182 ELISA Kit, pak2 ELISA Kit, cb422 ELISA Kit, kinase ELISA Kit, zgc:91798 ELISA Kit, wu:fb71h01 ELISA Kit, si:dz198m22.1 ELISA Kit, pak65 ELISA Kit, pakgamma ELISA Kit, PAKI ELISA Kit, Pak3 ELISA Kit, CH73-159L23.1 ELISA Kit, wu:fc52b03 ELISA Kit, zgc:113857 ELISA Kit, p21 (RAC1) activated kinase 2 ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2 ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2a ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2 S homeolog ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2b ELISA Kit, PAK2 ELISA Kit, Pak2 ELISA Kit, pak2a ELISA Kit, pak2.S ELISA Kit, pak2b ELISA Kit
    Gene ID
    5062
    UniProt
    Q13177
    Pathways
    MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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