EIF4E ELISA Kit

Catalog No. ABIN5526725

Product Details EIF4E ELISA Kit

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Binding Specificity: pSer209, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Purpose: Human Phospho-WNK1 (Thr60) and Total WNK1 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures eIF-4E phosphorylated at Serine-209 as well as total eIF-4E in adherent cell lines.

Sample Type: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes Human eIF-4E phosphorylated at site Serine-209 as well as total eIF-4E.

Characteristics:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Components:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: eIF-4E (EIF4E Products)

Synonyms: AUTS19 ELISA Kit, CBP ELISA Kit, EIF4E1 ELISA Kit, EIF4EL1 ELISA Kit, EIF4F ELISA Kit, eif4e-1 ELISA Kit, zgc:86680 ELISA Kit, EG668879 ELISA Kit, Eif4e-ps ELISA Kit, If4e ELISA Kit, eIF-4E ELISA Kit, 0260/09 ELISA Kit, 0587/11 ELISA Kit, 0589/11 ELISA Kit, 0919/12 ELISA Kit, 1004/13 ELISA Kit, 2 ELISA Kit, 7238 ELISA Kit, CG4035 ELISA Kit, CT13384 ELISA Kit, CT39424 ELISA Kit, CT39426 ELISA Kit, D-eIF4E ELISA Kit, Dmel\\CG4035 ELISA Kit, Eif4E ELISA Kit, Eif4e ELISA Kit, IF4E ELISA Kit, dEif4e ELISA Kit, deIF-4E ELISA Kit, deIF4E ELISA Kit, eIF 4E ELISA Kit, eIF-4E1 ELISA Kit, eIF-4E2 ELISA Kit, eIF-4EII ELISA Kit, eIF-4e ELISA Kit, eIF4E ELISA Kit, eIF4E-1 ELISA Kit, eIF4E-1/2 ELISA Kit, eIF4E-2 ELISA Kit, eIF4E1 ELISA Kit, eIF4EI ELISA Kit, eIF4e ELISA Kit, eif-4E ELISA Kit, eif-4e ELISA Kit, eif4e ELISA Kit, l(3)07238 ELISA Kit, l(3)67Af ELISA Kit, l(3)S025007 ELISA Kit, l(3)S026009 ELISA Kit, l(3)S058711 ELISA Kit, l(3)S091912 ELISA Kit, cbp ELISA Kit, eif4f ELISA Kit, eif4e1 ELISA Kit, eif4el1 ELISA Kit, EIF-4E ELISA Kit, zgc:101581 ELISA Kit, eukaryotic translation initiation factor 4E ELISA Kit, eukaryotic translation initiation factor 4ea ELISA Kit, Eukaryotic initiation factor 4E ELISA Kit, eucaryotic initiation factor 6 ELISA Kit, eukaryotic translation initiation factor 4E L homeolog ELISA Kit, eukaryotic translation initiation factor 4E family member 1B S homeolog ELISA Kit, translation initiation factor eIF4E ELISA Kit, eukaryotic translation initiation factor 4eb ELISA Kit, EIF4E ELISA Kit, eif4ea ELISA Kit, Eif4e ELISA Kit, eIF-4E ELISA Kit, eif6 ELISA Kit, eif4e ELISA Kit, eif4e.L ELISA Kit, eif4e1b.S ELISA Kit, LOC100533326 ELISA Kit, eif4eb ELISA Kit

Gene ID: 1977

UniProt: P06730

Pathways: BCR Signaling