PRKAA1 ELISA Kit

Catalog No. ABIN5526722

Product Details PRKAA1 ELISA Kit

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Binding Specificity: pSer487, total

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human, Mouse and Rat Phospho-AMPKa1 (Ser487) and Total AMPKa1 ELISA Kit. This assay semi-quantitatively measures AMPKa1 phosphorylated at Serine-487 as well as total AMPKa1 in cell lysate samples.

Sample Type: Cell Culture Lysate

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes Human, Mouse and Rat AMPKa1 phosphorylated at site Serine-487 as well as total AMPKa1.

Characteristics:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: AMPKa1 (PRKAA1 Products)

Synonyms: AMPK ELISA Kit, AMPKa1 ELISA Kit, cb116 ELISA Kit, im:7154392 ELISA Kit, sb:cb130 ELISA Kit, wu:fa94c10 ELISA Kit, AMPK1 ELISA Kit, AMPKalpha1 ELISA Kit, AI194361 ELISA Kit, AI450832 ELISA Kit, AL024255 ELISA Kit, C130083N04Rik ELISA Kit, ampk ELISA Kit, ampka1 ELISA Kit, PRKAA1 ELISA Kit, protein kinase AMP-activated catalytic subunit alpha 1 ELISA Kit, protein kinase, AMP-activated, alpha 1 catalytic subunit ELISA Kit, protein kinase, AMP-activated, alpha 1 catalytic subunit L homeolog ELISA Kit, PRKAA1 ELISA Kit, prkaa1 ELISA Kit, Prkaa1 ELISA Kit, prkaa1.L ELISA Kit

Gene ID: 5562

UniProt: Q13131, Q5EG47, P54645

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect