FOXO3 ELISA Kit

Catalog No. ABIN5526710

Product Details FOXO3 ELISA Kit

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Binding Specificity: pSer263, total

Reactivity: Human, Mouse

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Purpose: Cell-Based ELISA Kit. This assay semi-quantitatively measures FOXO3 phosphorylated at Serine-263 as well as total FOXO3 in adherent cell lines.

Sample Type: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes Human and Mouse FOXO3 phosphorylated at site Serine-263 as well as total FOXO3.

Characteristics:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Components:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FOXO3 (FOXO3 Products)

Synonyms: AF6q21 ELISA Kit, FKHRL1 ELISA Kit, FKHRL1P2 ELISA Kit, FOXO2 ELISA Kit, FOXO3A ELISA Kit, zgc:92176 ELISA Kit, wu:fb50g02 ELISA Kit, wu:fi33f07 ELISA Kit, Fkhrl1 ELISA Kit, Foxo3a ELISA Kit, FoxO3A ELISA Kit, 1110048B16Rik ELISA Kit, 2010203A17Rik ELISA Kit, C76856 ELISA Kit, Fkhr2 ELISA Kit, FOXO-3 ELISA Kit, FOXO3 ELISA Kit, foxo3a ELISA Kit, xfoxo3 ELISA Kit, foxO3 ELISA Kit, foxO ELISA Kit, fkhr ELISA Kit, foxo5 ELISA Kit, wu:fj65a12 ELISA Kit, zFkhr ELISA Kit, forkhead box O3 ELISA Kit, forkhead box O3A ELISA Kit, forkhead box O3 L homeolog ELISA Kit, forkhead box O3b ELISA Kit, FOXO3 ELISA Kit, foxo3a ELISA Kit, Foxo3 ELISA Kit, foxo3.L ELISA Kit, foxo3 ELISA Kit, foxO3 ELISA Kit, foxo3b ELISA Kit

Gene ID: 2309

UniProt: O43524, Q9WVH4

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis