SMAD1 ELISA Kit

Catalog No. ABIN5526702

Product Details SMAD1 ELISA Kit

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Binding Specificity: pSer463, pSer465, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Purpose: Cell-Based ELISA Kit. This assay semi-quantitatively measures SMAD1 phosphorylated at Serine-463/465 as well as total SMAD1 in adherent cell lines.

Sample Type: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes Human SMAD1 phosphorylated at site Serine-463/465 as well as total SMAD1.

Characteristics:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Components:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: SMAD1 (SMAD1 Products)

Synonyms: MLP ELISA Kit, Xmad1 ELISA Kit, Xsmad1 ELISA Kit, bsp1 ELISA Kit, jv4-1 ELISA Kit, jv41 ELISA Kit, madh1 ELISA Kit, madr1 ELISA Kit, smad-1 ELISA Kit, MADH1 ELISA Kit, fb39h09 ELISA Kit, wu:fb39h09 ELISA Kit, wu:fb97e04 ELISA Kit, 2/23 ELISA Kit, CG12399 ELISA Kit, Dmel\\CG12399 ELISA Kit, E(zen)2 ELISA Kit, En(vvl) ELISA Kit, MAD ELISA Kit, Mat ELISA Kit, P-Mad ELISA Kit, P-mad ELISA Kit, PMad ELISA Kit, Smad ELISA Kit, Smad1 ELISA Kit, apg ELISA Kit, c28 ELISA Kit, dMAD ELISA Kit, dMad ELISA Kit, l(2)K00237 ELISA Kit, l(2)k00237 ELISA Kit, mad ELISA Kit, p-Mad ELISA Kit, pMAD ELISA Kit, pMad ELISA Kit, pSmad ELISA Kit, BSP-1 ELISA Kit, BSP1 ELISA Kit, JV4-1 ELISA Kit, JV41 ELISA Kit, MADR1 ELISA Kit, AI528653 ELISA Kit, Madh1 ELISA Kit, Madr1 ELISA Kit, Mlp1 ELISA Kit, MusMLP ELISA Kit, smad1 ELISA Kit, xmad ELISA Kit, SMAD family member 1 ELISA Kit, Mothers against dpp ELISA Kit, SMAD family member 1 L homeolog ELISA Kit, smad1 ELISA Kit, SMAD1 ELISA Kit, Mad ELISA Kit, Smad1 ELISA Kit, smad1.L ELISA Kit

Gene ID: 4086

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development