GATA2 ELISA Kit

Catalog No. ABIN5526693

Product Details GATA2 ELISA Kit

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: DNA-Binding ELISA

Application: ELISA

Purpose: Human GATA-2 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA-2 binding sequences to semi-quantitatively detect active GATA-2 in lysates or nuclear extracts. Only available in North America.

Sample Type: Cell Lysate, Nuclear Extract

Analytical Method: Semi-Quantitative

Specificity: The olionucleotide/antibody pair provided in this kit recognizes human FRA-2 in whole lysates and nuclear extracts.

Characteristics:

• Specific transcription factor-DNA binding assay
• Perfect alternative to EMSA
• Easy to perform in an ELISA format
• Non-radioactive assay
• High throughput (96 well plate format)
• Assay can be completed within 5 hours

Components:

• 96-well Strip Microplate pre-coated with DNA probes
• DNA Binding Buffer
• Positive Control Sample
• Specific Competitor DNA probe
• Non-specific Competitor DNA probe
• Assay Reagent
• DTT
• Wash Buffer
• Primary Antibody
• HRP-conjugated Secondary Antibody
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions Absorbent paper
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details for GATA2

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Alternative Name: GATA-2 (GATA2 Products)

Synonyms: DCML ELISA Kit, MONOMAC ELISA Kit, NFE1B ELISA Kit, Gata-2 ELISA Kit, xgata-2 ELISA Kit, xgata2 ELISA Kit, GATA-2 ELISA Kit, NF-E1b ELISA Kit, GATA2 ELISA Kit, Gata2 ELISA Kit, gata2 ELISA Kit, zg2 ELISA Kit, zgc:91840 ELISA Kit, GATA binding protein 2 ELISA Kit, GATA binding protein 2 L homeolog ELISA Kit, amidase ELISA Kit, glutamyl/aspartyl-tRNA(Gln/Asp) amidotransferasesubunit A ELISA Kit, hypothetical protein ELISA Kit, glutamyl-tRNA(Gln) amidotransferase, subunit GatA (Glu-ADT subunit A) ELISA Kit, GATA2 transcription factor ELISA Kit, putative gata binding factor ELISA Kit, GATA binding protein 2a ELISA Kit, GATA binding protein 2b ELISA Kit, GATA2 ELISA Kit, Gata2 ELISA Kit, gata2.L ELISA Kit, RR_RS20245 ELISA Kit, gatA2 ELISA Kit, Smp_018590 ELISA Kit, gata2a ELISA Kit, gata2b ELISA Kit

Gene ID: 2624

UniProt: P23769

Pathways: Stem Cell Maintenance