eIF4EBP1 ELISA Kit

Catalog No. ABIN5526687

Product Details eIF4EBP1 ELISA Kit

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Binding Specificity: pThr36, total

Reactivity: Mouse, Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Purpose: Human and Mouse Phospho-4E-BP1 (Thr36) and Total 4E-BP1 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures 4E-BP1 phosphorylated at Threonine-36 as well as total 4E-BP1 in adherent cell lines.

Sample Type: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes Human and Mouse 4E-BP1 phosphorylated at site Threonine-36 as well as total 4E-BP1.

Characteristics:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Components:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: 4E-BP1 (EIF4EBP1 Products)

Synonyms: 4E-BP1 ELISA Kit, 4EBP1 ELISA Kit, BP-1 ELISA Kit, PHAS-I ELISA Kit, 4e-bp1 ELISA Kit, AA959816 ELISA Kit, 153432_at ELISA Kit, 43-BP ELISA Kit, 4E-BP ELISA Kit, 4EBP ELISA Kit, 4e-bp ELISA Kit, 4eBP ELISA Kit, 4ebp ELISA Kit, BcDNA.HL08053 ELISA Kit, BcDNA:HL08053 ELISA Kit, CG8846 ELISA Kit, CT8705 ELISA Kit, Dm 4E-BP ELISA Kit, Dmel\\CG8846 ELISA Kit, Phas1 ELISA Kit, Thor/EIF4EBP ELISA Kit, anon-WO0172774.158 ELISA Kit, d4E-BP ELISA Kit, d4E-BP1 ELISA Kit, d4EBP ELISA Kit, d4e-bp ELISA Kit, d4eBP ELISA Kit, dthor ELISA Kit, eIF4E-BP ELISA Kit, l(2)06270 ELISA Kit, l(2)k07736 ELISA Kit, l(2)k13517 ELISA Kit, pp20 ELISA Kit, thor ELISA Kit, EIF4EBP1 ELISA Kit, fc04h09 ELISA Kit, wu:fc04h09 ELISA Kit, zgc:64137 ELISA Kit, eukaryotic translation initiation factor 4E binding protein 1 ELISA Kit, CG8846 gene product from transcript CG8846-RA ELISA Kit, EIF4EBP1 ELISA Kit, Eif4ebp1 ELISA Kit, Thor ELISA Kit, eif4ebp1 ELISA Kit

Gene ID: 1978

UniProt: Q13541, Q60876

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling