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Pro-Cathepsin B ELISA Kit

Reactivity: Human Colorimetric Sandwich ELISA 61 pg/mL-15 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN5526685
  • Target See all Pro-Cathepsin B products
    Pro-Cathepsin B
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    61 pg/mL-15 ng/mL
    Minimum Detection Limit
    61 ng/mL
    Application
    ELISA
    Purpose
    Human Pro-Cathepsin B ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human Pro-Cathepsin B.
    Sensitivity
    61 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Reagent Preparation

    Recommended Dilution for serum and plasma samples4 fold

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Expiry Date
    6 months
  • Target See all Pro-Cathepsin B products
    Pro-Cathepsin B
    Abstract
    Pro-Cathepsin B Products
    Gene ID
    1508
    UniProt
    P07858
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