Pro-Cathepsin B ELISA Kit
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- Target See all Pro-Cathepsin B products
- Pro-Cathepsin B
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 61 pg/mL-15 ng/mL
- Minimum Detection Limit
- 61 ng/mL
- Application
- ELISA
- Purpose
- Human Pro-Cathepsin B ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Sample Type
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair recognizes Human Pro-Cathepsin B.
- Sensitivity
- 61 pg/mL
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Reagent Preparation
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Recommended Dilution for serum and plasma samples4 fold
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Expiry Date
- 6 months
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- Target See all Pro-Cathepsin B products
- Pro-Cathepsin B
- Abstract
- Pro-Cathepsin B Products
- Gene ID
- 1508
- UniProt
- P07858
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