PCK1 ELISA Kit
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- Target See all PCK1 ELISA Kits
- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Purpose
- For quantitative detection of PCK1 in serum, plasma, tissue homogenates.
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of PCK1. No significant cross-reactivity or interference between PCK1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between PCK1 and all the analogues, therefore, cross reaction may still exist.
- Sensitivity
- 0.094 ng/mL
- Components
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- ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Sandwich ELISA, Double Antibody
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
- For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
- Expiry Date
- 6 months
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- Target See all PCK1 ELISA Kits
- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
- Alternative Name
- PCK1 (PCK1 Products)
- Background
- PCK1(Phosphoenolpyruvate Carboxykinase 1)/PEPCK1/PEPCK-C/PEPCKC/PEPCK-CPEP carboxykinase/phosphoenolpyruvate carboxykinase 1(soluble)/phosphoenolpyruvate carboxykinase, cytosolic/phosphoenolpyruvate carboxykinase, cytosolic [GTP]/Phosphoenolpyruvate carbo
- UniProt
- P35558
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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