Neopterin ELISA Kit

Catalog No. ABIN5068146

Product Details Neopterin ELISA Kit

Target: Neopterin (NP)

Reactivity: Chemical

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Sample Type: Serum

Sensitivity: 0.2 ng/mL

Characteristics: Neopterin, Human, ELISA Kit

Components:

• Micrototer Plate, 1x96 wells
• Standard 0nM, 1x1.5ml
• Standard 1.35nM, 1x1.5ml
• Standard 4nM, 1x1.5ml
• Standard 12nM, 1x1.5ml
• Standard 37nM, 1x1.5ml
• Standard 111nM, 1x1.5ml
• Assay Buffer, 1x21ml
• Control 1, .3nM, 1x1.5ml
• Control 2, 15.nM, 1x1.5ml
• Neopterin (HRP Conjugate, 1x13ml
• Neopterin Antibody, 1x5ml
• Wash Buffer, 20X, 1x50ml
• TMB Substrate Solution, 1x19ml
• Stop Solution, 1N O4, 1x19ml

Application Details

Protocol: Principle:

• Neopterin ELISA kit is based on competitive binding of human Neopterin from serum samples and enzyme-labeled Neopterin to Neopterin specific antibodies immobilized on microtiter plates. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (blue color is inversely proportional to the amount of Neopterin present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow. Absorbance is then measured on an ELISA reader at 450nm and the concentration of Neopterin in samples and control is read off the standard curve.

Assay Precision: Precision CV: < 10% for Neopterin range 2-50nmol/L

• Inter-assay Precision CV: < 10% for Neopterin range 2-50nmol/L

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for Neopterin

Target: Neopterin (NP)

Alternative Name: Neopterin (NP Products)

Target Type: Chemical

Background: Neopterin [D-erythro-neopterin] belongs to the group of pteridines. Neopterin as well as other pteridines are derived in vivo from guanosine-triphophate (GTP). Neopterin represents a precursor molecule of biopterin that is an essential cofactor in neurotransmitter synthesis, and it is also involved in variety of oxidation-reduction reactions in the body. The enzyme GTP-cyclohydrolase-I catalyzes this reaction in monocytes and macrophages. Neopterin is exclusively released by activated macrophages, and can be determined in serum, plasma and other body fluids. Neopterin biosynthesis is closely associated with activation of the cellular immune system. Higher levels of neopterin were found in patients with viral infections. Antigenic stimulation of human peripheral blood mononuclear cells leads to neopterin release into cell culture medium. Gamma Interferon treatment of macrophages in vitro also increases neopterin levels and generates highly reactive oxygen species (ROS). Neopterin is capable of enhancing ONOO- - as well as Cu(II)-mediated LDL oxidation, whereas 7,8-dihydroneopterin mainly protects LDL from oxidation.