HAMA ELISA Kit

Catalog No. ABIN5068122

Product Details HAMA ELISA Kit

Target: HAMA (Human Anti-Mouse Antibody (HAMA))

Reactivity: Human, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Serum

Sensitivity: > 3 ng/mL

Characteristics: HAMA (Human anti-Mouse Antibody) ELISA Kit

Components:

• HAMA Microtiter Plate, Pre-coated with mouse IgG. 1x96 wells
• HRP Conjugate, Mouse IgG labeled with Horseradish Peroxidase (HRP). 1x12ml
• * HAMA Standard, Rabbit anti-Mouse IgG antibody, supplied as a lyophilized powder from a buffered protein base with preservative. 2x800ng
• Calibrator Diluent, Animal serum with buffer and preservative. 1x25ml
• Wash Buffer, 20X, 20-fold concentrated solution of buffered surfactant. 1x60ml
• Substrate A, Buffered solution with 2. 1x10ml
• Substrate B, Buffered solution with TMB. 1x10ml
• Stop Solution, 2N Sulphuric Acid (O4). 1x15ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• This assay applies an enzyme-linked immunosorbent assay (ELISA) technique. A purified mouse IgG has been pre-coated onto the wells of microplate. Standard (rabbit anti-mouse IgG) and specimens are added into the appropriate wells simultaneously with a HRP-labelled mouse IgG, and incubated. The Human Anti-Mouse Antibody (HAMA), if present, will bind with the mouse IgG which coated on the wells and HRP- labelled mouse IgG as well, forming a "Sandwich". Following a wash to remove any unbound substance, a substrate solution is added. The developed colour is directly proportional to the anti-mouse antibody concentration in the specimen and Standard samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for HAMA

Target: HAMA (Human Anti-Mouse Antibody (HAMA))

Alternative Name: HAMA (Human anti-Mouse Antibody) (HAMA Products)

Target Type: Antibody