PDGF-BB Homodimer ELISA Kit
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- Target
- PDGF-BB Homodimer
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- The Mouse Platelet-Derived Growth Factor-BB (PDGF-BB) ELISA Kit is a quantitative sandwich assay for the detection of PDGF-BB in mouse serum, plasma, tissue homogenates and other biological fluids.
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 23.438 pg/mL
- Characteristics
- PDGF-BB (Platelet-Derived Growth Factor-BB) ELISA Kit (Mouse)
- Components
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- *357966A: Microtiter Strips, 8x12 wells.
- *357966B: Standard, 2 vials
- 357966C: Sample/Standard Dilution Buffer, 1x20ml
- 357966D: Antibody-Biotin (Concentrated), 1x120ul
- 357966E: Antibody Dilution Buffer, 1x10ml
- 357966F: Streptavidin-HRP Conjugate (SABC), 1x120ul
- 357966G: SABC Dilution Buffer, 1x10ml
- 357966H: TMB Substrate, 1x10ml
- 357966J: Stop Solution, 1x10ml
- 357966K: Wash Buffer (25X), 1x30ml
- Featured
- Discover our best selling ELISA Kit
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- Plate
- Pre-coated
- Protocol
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Principle:
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDGF-BB. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to PDGF-BB. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDGF-BB, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDGF-BB in the sample is then determined by comparing the O.D. of the sample to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- 4°C/-20°C
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- Target
- PDGF-BB Homodimer
- Alternative Name
- PDGF-BB (Platelet-Derived Growth Factor-BB)
- Synonyms
- PDGF-2 ELISA Kit, PDGF2 ELISA Kit, SIS ELISA Kit, SSV ELISA Kit, c-sis ELISA Kit, PDGF-B ELISA Kit, Sis ELISA Kit, platelet derived growth factor subunit B ELISA Kit, platelet derived growth factor, B polypeptide ELISA Kit, PDGFB ELISA Kit, Pdgfb ELISA Kit
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