beta Amyloid ELISA Kit

Catalog No. ABIN5067940

Product Details beta Amyloid ELISA Kit

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Binding Specificity: AA 1-40

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: The Human beta Amyloid 1-40 (Hu A beta40) ELISA kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for the NH2-terminus of Hu b-amyloid has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known Hu b-amyloid 40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-40 b-amyloid sequence. This COOH- terminal sequence is created upon cleavage of the analyzed precursor. Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody. After washing, horseradish peroxidase-labeled anti-rabbit antibody (enzyme) is added. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu A beta40 present in the original specimen.

Sample Type: Cerebrospinal Fluid, Tissue Homogenate, Tissue Culture Medium

Analytical Method: Quantitative

Cross-Reactivity (Details): Calculated cross reactivity: Hu

Sensitivity: > 6 pg/mL

Characteristics: β-Amyloid, aa1-40, Human, ELISA Kit

Components:

• * beta Amyloid Standard, 1x1vial
• Standard Diluent Buffer, 1x60ml
• Microtiter Plate, 1x96 wells
• beta Amyloid , Pab 1x6ml
• IgG (HRP) (100X), 1x125ul
• HRP Diluent, 1x25ml
• Wash Buffer (25X), 1x100ml
• TMB, 1x25ml
• Stop Solution, 1x25ml

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C,-80 °C

Storage Comment: 4°C/-70°C

Target Details for beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Alternative Name: beta-Amyloid (Abeta Products)

Synonyms: AAA ELISA Kit, ABETA ELISA Kit, ABPP ELISA Kit, AD1 ELISA Kit, APPI ELISA Kit, CTFgamma ELISA Kit, CVAP ELISA Kit, PN-II ELISA Kit, PN2 ELISA Kit, aaa ELISA Kit, abeta ELISA Kit, abpp ELISA Kit, ad1 ELISA Kit, appi ELISA Kit, ctfgamma ELISA Kit, cvap ELISA Kit, pn2 ELISA Kit, Abeta ELISA Kit, Abpp ELISA Kit, Adap ELISA Kit, Ag ELISA Kit, Cvap ELISA Kit, E030013M08Rik ELISA Kit, betaApp ELISA Kit, amyloid beta precursor protein ELISA Kit, amyloid beta (A4) precursor protein ELISA Kit, APP ELISA Kit, app ELISA Kit, App ELISA Kit

Pathways: Inflammasome