Interleukin 35 ELISA Kit

Catalog No. ABIN5026966

Product Details Interleukin 35 ELISA Kit

Target: Interleukin 35 (IL35)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human IL-35 concentrations in serum, plasma and other biological fluids.

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Sensitivity: < 9.4 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human IL-35 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-35 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-35 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-35 can be calculated using the standard curve.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Target Details for Interleukin 35

Target: Interleukin 35 (IL35)

Alternative Name: IL-35 (Interleukin 35) (IL35 Products)