Bcl-2 ELISA Kit
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- Target See all Bcl-2 (BCL2) ELISA Kits
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156-10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of Human Bcl-2 concentrations in serum, plasma and other biological fluids.
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensitivity
- < 0.094 ng/mL
- Components
- plate, standard, diluent, antibodies
- Material not included
- pipettes, tubes, reader, incubator
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- Discover our best selling BCL2 ELISA Kit
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B-Cell CLL/lymphoma 2 (BCL2) ELISA Kit
BCL2 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
B-Cell CLL/lymphoma 2 (BCL2) ELISA KitBCL2 Reactivity: Rat Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
B-Cell CLL/lymphoma 2 (BCL2) ELISA KitBCL2 Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
B-Cell CLL/lymphoma 2 (BCL2) ELISA KitBCL2 Reactivity: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
B-Cell CLL/lymphoma 2 (BCL2) ELISA KitBCL2 Reactivity: Rabbit Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
B-Cell CLL/lymphoma 2 (BCL2) ELISA KitBCL2 Reactivity: Guinea Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- Sample Volume
- 100 μL
- Assay Time
- 3 - 4 h
- Plate
- Pre-coated
- Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human Bcl-2 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human Bcl-2 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Bcl-2 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human Bcl-2 can be calculated using the standard curve.
- Restrictions
- For Research Use only
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- Precaution of Use
- protect your eyes
- Storage
- 4 °C
-
- Target See all Bcl-2 (BCL2) ELISA Kits
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
- Alternative Name
- Bcl -2 (B cell Lymphoma 2) (BCL2 Products)
- Synonyms
- Bcl-2 ELISA Kit, PPP1R50 ELISA Kit, bcl-2 ELISA Kit, AW986256 ELISA Kit, C430015F12Rik ELISA Kit, D630044D05Rik ELISA Kit, D830018M01Rik ELISA Kit, BCL-2 ELISA Kit, Bcl2 ELISA Kit, BCL2, apoptosis regulator ELISA Kit, B cell leukemia/lymphoma 2 ELISA Kit, B-cell CLL/lymphoma 2a ELISA Kit, B-cell CLL/lymphoma 2 protein ELISA Kit, BCL2 ELISA Kit, Bcl2 ELISA Kit, bcl2a ELISA Kit, BCL-2 ELISA Kit
- Pathways
- MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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