TNF alpha ELISA Kit
-
- Target See all TNF alpha ELISA Kits
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 15.6-1000 pg/mL
- Minimum Detection Limit
- 15.6 pg/mL
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of Human TNFAlpha concentrations in serum, plasma and other biological fluids.
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensitivity
- < 9.4 pg/mL
- Components
- plate, standard, diluent, antibodies
- Material not included
- pipettes, tubes, reader, incubator
- Featured
- Discover our best selling TNF alpha ELISA Kit
- Top Product
- Discover our top product TNF alpha ELISA Kit
-
-
- Sample Volume
- 100 μL
- Assay Time
- 3 - 4 h
- Plate
- Pre-coated
- Protocol
- This assay employs an antibody specific for Anti-human TNFAlpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFAlpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNFAlpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFAlpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Restrictions
- For Research Use only
-
- Precaution of Use
- protect your eyes
- Storage
- 4 °C
-
- Target See all TNF alpha ELISA Kits
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Alternative Name
- TNFalpha (Tumor Necrosis Factor Alpha) (TNF alpha Products)
- Synonyms
- DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit, RATTNF ELISA Kit, Tnfa ELISA Kit, tnf ELISA Kit, TNF-a ELISA Kit, TNFalpha ELISA Kit, Tnfsf1a ELISA Kit, TNFa ELISA Kit, cTNF ELISA Kit, Tnf-alpha ELISA Kit, tnfa-like ELISA Kit, TNF-ALPHA ELISA Kit, dif ELISA Kit, tnfa ELISA Kit, xtnf ELISA Kit, tnfsf2 ELISA Kit, tnf-alpha ELISA Kit, Cachectin ELISA Kit, tumor necrosis factor ELISA Kit, tumor necrosis factor b (TNF superfamily, member 2) ELISA Kit, tumor necrosis factor alpha ELISA Kit, tumor necrosis factor a (TNF superfamily, member 2) ELISA Kit, TNF ELISA Kit, Tnf ELISA Kit, tnf ELISA Kit, tnfb ELISA Kit, tnf-alpha ELISA Kit, LOC103694380 ELISA Kit, tnfa ELISA Kit
- Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
-