PD-1 ELISA Kit
-
- Target See all PD-1 (PDCD1) ELISA Kits
- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156-10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of Human PDL1 concentrations in serum, plasma and other biological fluids. This kit is for Research use only. Not for use in diagnostic/therapeutics procedures.
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensitivity
- < 0.094 ng/mL
- Components
- plate, standard, diluent, antibodies
- Material not included
- pipettes, tubes, reader, incubator
- Featured
- Discover our best selling PDCD1 ELISA Kit
- Top Product
- Discover our top product PDCD1 ELISA Kit
-
Programmed Cell Death 1 (PDCD1) ELISA Kit
PDCD1 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 Reactivity: Various Species Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 Reactivity: Human AA 25-167 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 Reactivity: Human Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 Reactivity: Human Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant
-
- Sample Volume
- 100 μL
- Assay Time
- 3 - 4 h
- Plate
- Pre-coated
- Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human PD-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human PD-1 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PD-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human PD-1 can be calculated using the standard curve.
- Restrictions
- For Research Use only
-
- Precaution of Use
- protect your eyes
- Storage
- 4 °C
-
- Target See all PD-1 (PDCD1) ELISA Kits
- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
- Alternative Name
- PD-1 (Programmed Cell Death Protein1) (PDCD1 Products)
- Synonyms
- CD279 ELISA Kit, PD-1 ELISA Kit, PD1 ELISA Kit, SLEB2 ELISA Kit, hPD-1 ELISA Kit, hPD-l ELISA Kit, Ly101 ELISA Kit, Pdc1 ELISA Kit, programmed cell death 1 ELISA Kit, PDCD1 ELISA Kit, Pdcd1 ELISA Kit
- Pathways
- Cancer Immune Checkpoints
-
