ALOX15 ELISA Kit

Catalog No. ABIN5026944

Human ALOX15 ELISA Kit Colorimetric assay for quantification of Human ALOX15.

Quick Overview for ALOX15 ELISA Kit (ABIN5026944)

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details ALOX15 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human 15-LO concentrations in serum, plasma and other biological fluids. This kit is for Research Use Only. Not for use in diagnostic/therapeutics procedures.

Analytical Method: Quantitative

Sensitivity: < 37.5 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human 15-LO coated on a 96-well plate. Standards and samples are pipetted into the wells and 15-LO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human 15-LO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of 15-LO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Target Details for ALOX15

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Alternative Name: 15-LO (Arachidonate 15-Lipoxygenase)

Pathways: Regulation of Actin Filament Polymerization