EIF2A ELISA Kit

Catalog No. ABIN4993426

Product Details EIF2A ELISA Kit

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.13 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.13 pg/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EIF2a in human serum, plasma and other biological fluids

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This kit recognizes natural and recombinantHumanEIF2a. No significant cross-reactivity or interference between HumanEIF2a and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between HumanEIF2a and all the analogues.

Sensitivity: 46.88 pg/mL

Components: Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis

Material not included: Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Application Details

Comment:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Sample Volume: 100 µL

Plate: Pre-coated

Protocol: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EIF2a. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for EIF2aand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain EIF2a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of EIF2a.You can calculate the concentration of EIF2ain the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4

Restrictions: For Research Use only

Handling

Handling Advice: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Storage: 4 °C/-20 °C

Storage Comment: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: Eukaryotic Translation Initiation Factor 2 Alpha (EIF2a) (EIF2A Products)

Synonyms: ATEIF2-A2 ELISA Kit, EIF2-A2 ELISA Kit, eukaryotic translation initiation factor 2 alpha subunit ELISA Kit, EIF-2A ELISA Kit, MST089 ELISA Kit, MSTP004 ELISA Kit, MSTP089 ELISA Kit, D030048D22 ELISA Kit, D3Ertd194e ELISA Kit, im:7145971 ELISA Kit, zgc:110460 ELISA Kit, DDBDRAFT_0185936 ELISA Kit, DDBDRAFT_0234177 ELISA Kit, DDB_0185936 ELISA Kit, DDB_0234177 ELISA Kit, Eif2a ELISA Kit, eif2a ELISA Kit, eif2alpha ELISA Kit, EIF2A ELISA Kit, CG9946 ELISA Kit, Dmel\\CG9946 ELISA Kit, Eif2alpha ELISA Kit, IF2A_DROME ELISA Kit, M(1)14C ELISA Kit, M(1)19-153 ELISA Kit, M(1)8e3-10 ELISA Kit, deIF2alpha ELISA Kit, eIF-2 ELISA Kit, eIF-2 a ELISA Kit, eIF-2 alpha ELISA Kit, eIF2 alpha ELISA Kit, eIF2-alpha ELISA Kit, eIF2a ELISA Kit, eIF2alpha ELISA Kit, eiF-2alpha ELISA Kit, elF2alpha ELISA Kit, l(1)14Cf ELISA Kit, l(1)19-153 ELISA Kit, eukaryotic translation initiation factor 2 alpha subunit ELISA Kit, eukaryotic translation initiation factor 2A ELISA Kit, eukaryotic translation initiation factor 2A, 65kDa ELISA Kit, eukaryotic translation initiation factor 2A S homeolog ELISA Kit, eukaryotic translation initiation factor ELISA Kit, eukaryotic translation Initiation Factor 2alpha ELISA Kit, EIF2 ALPHA ELISA Kit, EIF2A ELISA Kit, Eif2a ELISA Kit, eif2a ELISA Kit, eIF2a ELISA Kit, eif2a.S ELISA Kit, eif2A ELISA Kit, eIF-2alpha ELISA Kit

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly