CDK4 ELISA Kit

Catalog No. ABIN4993370

Product Details CDK4 ELISA Kit

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CDK4 in Mouse serum, plasma and other biological fluids

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This kit recognizes natural and recombinantMouseCDK4. No significant cross-reactivity or interference between MouseCDK4 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between MouseCDK4 and all the analogues.

Sensitivity: 37.5 pg/mL

Components: Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis

Material not included: Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Application Details

Comment:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Sample Volume: 100 µL

Plate: Pre-coated

Protocol: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CDK4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CDK4and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CDK4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CDK4.You can calculate the concentration of CDK4in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4

Restrictions: For Research Use only

Handling

Handling Advice: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Storage: 4 °C/-20 °C

Storage Comment: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: Cyclin Dependent Kinase 4 (CDK4) (CDK4 Products)

Synonyms: zgc:153726 ELISA Kit, CMM3 ELISA Kit, PSK-J3 ELISA Kit, Crk3 ELISA Kit, 8-6 ELISA Kit, CDK4 ELISA Kit, CDK4/6 ELISA Kit, CG5072 ELISA Kit, Cdk4/6 ELISA Kit, DmCdk4 ELISA Kit, Dmel\\CG5072 ELISA Kit, Pk53C ELISA Kit, Pk?7 ELISA Kit, cdk ELISA Kit, cdk4 ELISA Kit, cdk4/6 ELISA Kit, dCdk4 ELISA Kit, l(2)05428 ELISA Kit, l(2)0671 ELISA Kit, l(2)k06503 ELISA Kit, l(2)s4639 ELISA Kit, l(2)sh0671 ELISA Kit, xcdk4 ELISA Kit, cyclin-dependent kinase 4 ELISA Kit, cyclin dependent kinase 4 ELISA Kit, Cyclin-dependent kinase 4 ELISA Kit, cyclin-dependent kinase 4 L homeolog ELISA Kit, cdk4 ELISA Kit, CDK4 ELISA Kit, Cdk4 ELISA Kit, cdk4.L ELISA Kit

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size