15d-PGJ2 ELISA Kit

Catalog No. ABIN4993245

Product Details 15d-PGJ2 ELISA Kit

Target: 15d-PGJ2 (15-Deoxy-Prostaglandin J2 (15d-PGJ2))

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 3.13 ng/mL - 200 ng/mL

Minimum Detection Limit: 3.13 ng/mL

Application: ELISA

Purpose: The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of 15d-PGJ2 in serum, plasma and other biological fluids

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This kit recognizes natural and recombinant 15d-PGJ2. No signif icant cross-reactivity or interference between 15d-PGJ2 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between 15d-PGJ2 and all the analogues.

Sensitivity: 1.88 ng/mL

Components: Micro ELISA Plate(Dismountable)
Reference Standard
Concentrated Biotinylated Detection Ab (100×)
Concentrated HRP Conjugate (100×)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis

Material not included: Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Application Details

Comment:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Sample Volume: 50 µL

Plate: Pre-coated

Protocol: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 15d-PGJ2. During the reaction, 15d-PGJ2 in the sample or standard competes with a fixed amount of 15d-PGJ2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 15d-PGJ2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 15d-PGJ2 in the samples is then determined by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4

Restrictions: For Research Use only

Handling

Handling Advice: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Storage: 4 °C/-20 °C

Storage Comment: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Target Details for 15d-PGJ2

Target: 15d-PGJ2 (15-Deoxy-Prostaglandin J2 (15d-PGJ2))

Alternative Name: 15-Deoxy-Delta12,14-prostaglandin J2 (15d-PGJ2) (15d-PGJ2 Products)

Target Type: Hormone