Cytochrome C ELISA Kit

Catalog No. ABIN4986817

Product Details Cytochrome C ELISA Kit

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 39-2500 pg/mL

Minimum Detection Limit: 39 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Natural and recombinant Human Cytochrome C Ligand

Sensitivity: 10 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: Cytochrome C (CYCS Products)

Synonyms: CYC ELISA Kit, HCS ELISA Kit, THC4 ELISA Kit, cyct ELISA Kit, cyc ELISA Kit, hcs ELISA Kit, CYCS ELISA Kit, cytc ELISA Kit, CYCSA ELISA Kit, CG17903 ELISA Kit, Cyt C ELISA Kit, Cyt c ELISA Kit, Cyt-c2 ELISA Kit, CytC-2 ELISA Kit, Cytc ELISA Kit, Cytc-p ELISA Kit, DC4 ELISA Kit, DMc01 ELISA Kit, Dmel\\CG17903 ELISA Kit, cyt c ELISA Kit, cyt c-p ELISA Kit, cyt-c-p ELISA Kit, cyt-c2 ELISA Kit, cyt.c ELISA Kit, cytochrome c ELISA Kit, dc4 ELISA Kit, pDMc01 ELISA Kit, wu:fk52b01 ELISA Kit, zgc:86706 ELISA Kit, ENSMUSG00000058927 ELISA Kit, cytochrome c, somatic ELISA Kit, cytochrome c, somatic S homeolog ELISA Kit, Cytochrome c proximal ELISA Kit, cytochrome c, somatic b ELISA Kit, cytochrome c, somatic pseudogene ELISA Kit, cytochrome c-like ELISA Kit, cytochrome c ELISA Kit, CYCS ELISA Kit, cycs.S ELISA Kit, cycs ELISA Kit, Cycs ELISA Kit, Cyt-c-p ELISA Kit, cycsb ELISA Kit, Gm10053 ELISA Kit, LOC100724240 ELISA Kit, CYC ELISA Kit, LOC100847700 ELISA Kit, LOC101107954 ELISA Kit, LOC106829744 ELISA Kit, LOC106992390 ELISA Kit, LOC102085712 ELISA Kit

Background: Human somatic cytochrome c is a 15 kDa, 104 amino acid (aa) polypeptide that participates in both oxidative phosphorylation and apoptosis (1). It exhibits 91 % aa identity with mouse/rat cytochrome c (1-3). The molecule is initially synthesized in the cytoplasm as an extended, 12 kDa apoprotein. This molecule is subsequently transported across the outer mitochondrial membrane into the intermitochondrial space via a receptor-independent process. Here, it associates with an inner mitochondrial membrane enzyme called CCHL (cytochrome c heme lyase) which covalently attaches one heme molecule to the N-terminus, forming functional cytochrome c (4-6). This covalent attachment causes cytochrome c to undergo a conformational change to a globular molecule effectively trapping cytochrome c in the intermitochrondrial space.Within the intermitochondrial space, cytochrome c is actively involved in the oxidative phosphorylation pathway. It transports electrons from the cytochrome c reductase complex to the cytochrome c oxidase complex (7-9). This transports excess electrons along the respiratory pathway and generates ATP for energy-dependent processes. Alternatively, and in response to apoptotic signals, cytochrome c can be released from mitochondria into the cytosol. Here, it activates an apoptotic program via one of many possible caspase-driven cascades (8, 10-12). The events which trigger an apoptotic signal (such as growth factor withdrawal) are not well understood. The result of such a signal, however, has been suggested to be a translocation of cytosolic Bax and/or Bad to the outer mitochondrial membrane where they overcome a Bcl-2-mediated stabilization (13-15). Bax-mediated destabilization may take the form of channel creation or mitochondrial swelling, resulting in cytochrome c release (15-18). Whatever the mechanism, released cytochrome c participates in the formation of a cytosolic complex which is composed of 15 kDa cytochrome c, 130 kDa Apaf-1 (apoptotic protease activating factor-1), dATP, and 46 kDa Apaf-3/caspase-9 (15, 18, 19). Within this complex, Apaf-3/caspase-9 is activated, leading to the downstream activation of caspases-3,-7 and -9, followed by additional caspases that ultimately lead to cellular apoptosis (10, 18, 20,21).

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity