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SMAD5 ELISA Kit

SMAD5 Reactivity: Human pSer463 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN4886300
  • Target See all SMAD5 ELISA Kits
    SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))
    Binding Specificity
    pSer463, pSer465, total
    Reactivity
    • 3
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-SMAD5 (SER463/465) and Total SMAD5 ELISA Kit. This assay semi-quantitatively measures phophorylated SMAD5 (Ser463/465) and Total SMAD5 in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Human SMAD5 phosphorylated at site Serine-463/465 and total SMAD5.
    Characteristics
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all SMAD5 ELISA Kits
    SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))
    Alternative Name
    SMAD5 (SMAD5 Products)
    Synonyms
    DWFC ELISA Kit, JV5-1 ELISA Kit, MADH5 ELISA Kit, Madh5 ELISA Kit, SBN ELISA Kit, fb67b04 ELISA Kit, mad ELISA Kit, madh5 ELISA Kit, sb:cb400 ELISA Kit, smd5 ELISA Kit, wu:fb67b04 ELISA Kit, SMAD5 ELISA Kit, DKFZp468M0619 ELISA Kit, 1110051M15Rik ELISA Kit, AI451355 ELISA Kit, Dwf-C ELISA Kit, MusMLP ELISA Kit, SMAD family member 5 ELISA Kit, SMAD5 ELISA Kit, Smad5 ELISA Kit, smad5 ELISA Kit
    Gene ID
    4090
    UniProt
    Q99717
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