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RPS6KA3 ELISA Kit

RPS6KA3 Reactivity: Human, Mouse, Rat pSer386 Colorimetric Sandwich ELISA Cell Culture Lysate
Catalog No. ABIN4886276
  • Target See all RPS6KA3 ELISA Kits
    RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))
    Binding Specificity
    pSer386, total
    Reactivity
    Human, Mouse, Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human, Mouse and Rat Phospho-RSK2 (Ser386) and Total RSK2 ELISA Kit. This assay semi-quantitatively measures phophorylated RSK2 (Ser386) and Total RSK2 in lysate samples.
    Sample Type
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    This ELISA kit recognizes Human, Mouse and Rat RSK2 phosphorylated at site Serine-386 as well as total RSK2.
    Characteristics
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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    Discover our top product RPS6KA3 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all RPS6KA3 ELISA Kits
    RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))
    Alternative Name
    RSK2 (RPS6KA3 Products)
    Synonyms
    HU-1 ELISA Kit, MAPKAPK1A ELISA Kit, RSK ELISA Kit, RSK1 ELISA Kit, CLS ELISA Kit, HU-3 ELISA Kit, ISPK-1 ELISA Kit, MAPKAPK1B ELISA Kit, MRX19 ELISA Kit, RSK2 ELISA Kit, S6K-alpha3 ELISA Kit, p90-RSK2 ELISA Kit, pp90RSK2 ELISA Kit, MPK-9 ELISA Kit, Rsk2 ELISA Kit, RGD1563860 ELISA Kit, id:ibd1103 ELISA Kit, rps6ka3 ELISA Kit, si:ch211-114c12.12 ELISA Kit, wu:fk86e02 ELISA Kit, zgc:56006 ELISA Kit, zgc:158386 ELISA Kit, ribosomal protein S6 kinase A1 ELISA Kit, ribosomal protein S6 kinase A3 ELISA Kit, ribosomal protein S6 kinase polypeptide 3 ELISA Kit, ribosomal protein S6 kinase a, polypeptide 3a ELISA Kit, ribosomal protein S6 kinase, polypeptide 3b ELISA Kit, RPS6KA1 ELISA Kit, RPS6KA3 ELISA Kit, Rps6ka3 ELISA Kit, rps6ka3a ELISA Kit, rps6ka3b ELISA Kit
    Gene ID
    6197
    UniProt
    P51812, P18654, D3Z8E0
    Pathways
    MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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