JAK1 ELISA Kit
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- Target See all JAK1 ELISA Kits
- JAK1 (Janus Kinase 1 (JAK1))
- Binding Specificity
- pThr246, total
- Reactivity
- Human, Rat, Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Human Phospho-PRAS40 (T246) & Total PRAS40 ELISA Kit. This assay semi-quantitatively measures phophorylated PRAS40 (Thr246) & Total PRAS40 in lysate samples.
- Sample Type
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- Specificity
- The antibody pair provided in this kit recognizes human JAK1 phosphorylated at site Tyrosine-1022 and total JAK1
- Characteristics
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Material not included
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product JAK1 ELISA Kit
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Expiry Date
- 6 months
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-
Lutein modulates transcription dysregulation of adhesion molecules and spermatogenesis transcription factors induced by testicular ischemia reperfusion injury: it could be SAFE." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 389, Issue 5, pp. 539-51, (2017) (PubMed).
: "
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Lutein modulates transcription dysregulation of adhesion molecules and spermatogenesis transcription factors induced by testicular ischemia reperfusion injury: it could be SAFE." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 389, Issue 5, pp. 539-51, (2017) (PubMed).
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- Target See all JAK1 ELISA Kits
- JAK1 (Janus Kinase 1 (JAK1))
- Alternative Name
- JAK1 (JAK1 Products)
- Synonyms
- JAK1A ELISA Kit, JAK1B ELISA Kit, JTK3 ELISA Kit, AA960307 ELISA Kit, C130039L05Rik ELISA Kit, wu:fb93e10 ELISA Kit, jak1 ELISA Kit, Janus kinase 1 ELISA Kit, tyrosine-protein kinase JAK1 ELISA Kit, JAK1 ELISA Kit, Jak1 ELISA Kit, jak1 ELISA Kit, LOC100286690 ELISA Kit
- Gene ID
- 3716
- UniProt
- P23458
- Pathways
- JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Hepatitis C, Toll-Like Receptors Cascades, Unfolded Protein Response
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