Insulin Receptor ELISA Kit

Catalog No. ABIN4886144

Product Details Insulin Receptor ELISA Kit

Target: Insulin Receptor (INSR)

Binding Specificity: pTyr1189, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Phospho-INSR (Y1189) and Total INSR ELISA Kit. This assay semi-quantitatively measures phophorylated INSR (Tyr1189) and Total INSR in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes Human INSR phosphorylated at site Tyrosine-1189 and total INSR.

Characteristics:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: Insulin R (INSR Products)

Synonyms: CD220 ELISA Kit, HHF5 ELISA Kit, 4932439J01Rik ELISA Kit, D630014A15Rik ELISA Kit, IR ELISA Kit, IR-A ELISA Kit, IR-B ELISA Kit, 18402 ELISA Kit, CG18402 ELISA Kit, DIHR ELISA Kit, DILR ELISA Kit, DIR ELISA Kit, DIRH ELISA Kit, DIRbeta ELISA Kit, DInR ELISA Kit, DInr ELISA Kit, Dir-a ELISA Kit, Dir-b ELISA Kit, Dmel\\CG18402 ELISA Kit, INR ELISA Kit, INS ELISA Kit, Inr ELISA Kit, Inr-alpha ELISA Kit, Inr-beta ELISA Kit, InsR ELISA Kit, dINR ELISA Kit, dIR ELISA Kit, dIRH ELISA Kit, dInR ELISA Kit, dInr ELISA Kit, dInsR ELISA Kit, dinr ELISA Kit, dir ELISA Kit, er10 ELISA Kit, inr ELISA Kit, insulin/insulin-like growth factor receptor ELISA Kit, l(3)05545 ELISA Kit, l(3)93Dj ELISA Kit, l(3)er10 ELISA Kit, lnR ELISA Kit, ir-A ELISA Kit, CTK-1 ELISA Kit, ir ELISA Kit, INSR ELISA Kit, NV14476 ELISA Kit, cd220 ELISA Kit, hhf5 ELISA Kit, insulin receptor ELISA Kit, Insulin-like receptor ELISA Kit, insulin receptor L homeolog ELISA Kit, INSR ELISA Kit, Insr ELISA Kit, InR ELISA Kit, LOC100122567 ELISA Kit, LOC100451802 ELISA Kit, insr.L ELISA Kit

Gene ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity