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GSK3 alpha ELISA Kit

GSK3a Reactivity: Human, Rat, Mouse pSer21 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN4886114
  • Target See all GSK3 alpha (GSK3a) ELISA Kits
    GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))
    Binding Specificity
    pSer21
    Reactivity
    • 7
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human/Mouse/Rat Phospho-GSK3a (S21) ELISA Kit. This assay semi-quantitatively measures phophorylated GSK3a (Ser21) in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human Phospho-GSK3 alpha (pSer21).
    Characteristics
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all GSK3 alpha (GSK3a) ELISA Kits
    GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))
    Alternative Name
    GSK3a (GSK3a Products)
    Synonyms
    2700086H06Rik ELISA Kit, GSK3A ELISA Kit, GSK-3[a] ELISA Kit, GSK-3[b] ELISA Kit, GSK3 ELISA Kit, gsk3a ELISA Kit, xgsk3 ELISA Kit, glycogen synthase kinase 3 alpha ELISA Kit, glycogen synthase kinase 3 alpha b ELISA Kit, glycogen synthase kinase 3 alpha S homeolog ELISA Kit, GSK3A ELISA Kit, Gsk3a ELISA Kit, gsk3a ELISA Kit, gsk3ab ELISA Kit, gsk3a.S ELISA Kit, Bm1_31380 ELISA Kit
    Gene ID
    2931
    UniProt
    P49840
    Pathways
    PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect
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