FGFR1 ELISA Kit

Catalog No. ABIN4886099

Product Details FGFR1 ELISA Kit

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Binding Specificity: pTyr

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Phosphotyrosine FGFR1 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine FGFR1 in lysate samples.

Sample Type: Cell Culture Lysate

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes human FGFR1 phosphotyrosine.

Characteristics:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for FGFR1

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Alternative Name: FGFR1 (FGFR1 Products)

Synonyms: FGFR ELISA Kit, fgfr ELISA Kit, X1FGFR ELISA Kit, XFGFR-1 ELISA Kit, XFGFRA2 ELISA Kit, bfgfr ELISA Kit, cek ELISA Kit, fgfr1 ELISA Kit, flg ELISA Kit, flt-2 ELISA Kit, flt2 ELISA Kit, kal2 ELISA Kit, ogd ELISA Kit, xfgfr1 ELISA Kit, CG7223 ELISA Kit, CT22273 ELISA Kit, CT39172 ELISA Kit, DFGF-R1 ELISA Kit, DFGF-R2 ELISA Kit, DFR-1 ELISA Kit, DFR1 ELISA Kit, DFR1/DFGF-R2 ELISA Kit, DPR3 ELISA Kit, DTRK(FR1) ELISA Kit, Dfr-1 ELISA Kit, Dfr1 ELISA Kit, DmHD-38 ELISA Kit, Dmel\\CG7223 ELISA Kit, Dtk1 ELISA Kit, EMS2 ELISA Kit, FGF-R2 ELISA Kit, FGFR2 ELISA Kit, FR1 ELISA Kit, Fr1 ELISA Kit, HD-38 ELISA Kit, HTL/FGFR1 ELISA Kit, Htl ELISA Kit, Tk1 ELISA Kit, dtk1 ELISA Kit, i100 ELISA Kit, i150 ELISA Kit, i79 ELISA Kit, j372 ELISA Kit, cd331 ELISA Kit, fgfbr ELISA Kit, fgfr-1 ELISA Kit, hbgfr ELISA Kit, n-sam ELISA Kit, ach ELISA Kit, cd333 ELISA Kit, cek2 ELISA Kit, fgfr-3 ELISA Kit, hsfgfr3ex ELISA Kit, jtk4 ELISA Kit, BFGFR ELISA Kit, CD331 ELISA Kit, CEK ELISA Kit, FGFBR ELISA Kit, FGFR-1 ELISA Kit, FLG ELISA Kit, FLT-2 ELISA Kit, FLT2 ELISA Kit, HBGFR ELISA Kit, HH2 ELISA Kit, KAL2 ELISA Kit, N-SAM ELISA Kit, OGD ELISA Kit, bFGF-R-1 ELISA Kit, AW208770 ELISA Kit, Eask ELISA Kit, Fgfr-1 ELISA Kit, Flt-2 ELISA Kit, Hspy ELISA Kit, FLG-1 ELISA Kit, FGFRI ELISA Kit, x1fgfr ELISA Kit, cb231 ELISA Kit, sb:cb231 ELISA Kit, fibroblast growth factor receptor ELISA Kit, fibroblast growth factor receptor 1 S homeolog ELISA Kit, basic fibroblast growth factor receptor 1 ELISA Kit, heartless ELISA Kit, fibroblast growth factor receptor 1 ELISA Kit, fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) L homeolog ELISA Kit, Fibroblast growth factor receptor 1 ELISA Kit, fibroblast growth factor receptor 1 L homeolog ELISA Kit, fibroblast growth factor receptor 1a ELISA Kit, FGFR ELISA Kit, fgfr ELISA Kit, fgfr1.S ELISA Kit, CpipJ_CPIJ005849 ELISA Kit, htl ELISA Kit, fgfr1 ELISA Kit, fgfr3.L ELISA Kit, FGFR1 ELISA Kit, Fgfr1 ELISA Kit, fgfr1.L ELISA Kit, fgfr1a ELISA Kit

Gene ID: 2260

UniProt: P11362

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, S100 Proteins