EPH Receptor B1 ELISA Kit

Catalog No. ABIN4886074

Product Details EPH Receptor B1 ELISA Kit

Target: EPH Receptor B1 (EPHB1)

Binding Specificity: pTyr

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Phosphotyrosine EphB1 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine EphB1 in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-EphB1.

Characteristics:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for EPH Receptor B1

Target: EPH Receptor B1 (EPHB1)

Alternative Name: EphB1 (EPHB1 Products)

Synonyms: ELK ELISA Kit, EPHT2 ELISA Kit, Hek6 ELISA Kit, NET ELISA Kit, ephb1-a ELISA Kit, xek ELISA Kit, Xek ELISA Kit, MGC89790 ELISA Kit, EPHB1 ELISA Kit, CEK6 ELISA Kit, EK6 ELISA Kit, 9330129L11 ELISA Kit, AW488255 ELISA Kit, C130099E04Rik ELISA Kit, Cek6 ELISA Kit, ENSMUSG00000074119 ELISA Kit, Elk ELISA Kit, Elkh ELISA Kit, Net ELISA Kit, Ephb2 ELISA Kit, Erk ELISA Kit, elk ELISA Kit, EPH receptor B1 ELISA Kit, EPH receptor B1 S homeolog ELISA Kit, ephrin type-B receptor 1 ELISA Kit, Eph receptor B1 ELISA Kit, EPHB1 ELISA Kit, ephb1.S ELISA Kit, ephb1 ELISA Kit, LOC100467687 ELISA Kit, Ephb1 ELISA Kit

Gene ID: 2047

UniProt: P54762

Pathways: RTK Signaling