EPH Receptor A6 ELISA Kit
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- Target See all EPH Receptor A6 (Epha6) ELISA Kits
- EPH Receptor A6 (Epha6)
- Binding Specificity
- pTyr
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Custom Human Phosphotyrosine EphA6 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine EphA6 in lysate samples.
- Sample Type
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- Specificity
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-EphA6.
- Characteristics
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Expiry Date
- 6 months
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- Target See all EPH Receptor A6 (Epha6) ELISA Kits
- EPH Receptor A6 (Epha6)
- Alternative Name
- EphA6 (Epha6 Products)
- Synonyms
- EPHA6 ELISA Kit, Ehk2 ELISA Kit, Hek12 ELISA Kit, m-ehk2 ELISA Kit, EHK-2 ELISA Kit, EHK2 ELISA Kit, EK12 ELISA Kit, EPA6 ELISA Kit, HEK12 ELISA Kit, PRO57066 ELISA Kit, EPH receptor A6 ELISA Kit, Eph receptor A6 ELISA Kit, EPHA6 ELISA Kit, epha6 ELISA Kit, Epha6 ELISA Kit
- Gene ID
- 285220
- UniProt
- Q9UF33
- Pathways
- RTK Signaling
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