DDR2 ELISA Kit

Catalog No. ABIN4886038

Product Details DDR2 ELISA Kit

Target: DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))

Binding Specificity: pTyr740, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Phospho-DDR2 (Tyr740) and Total DDR2 ELISA Kit. This assay semi-quantitatively measures phophorylated DDR2 (Tyr740) and Total DDR2 in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes Human DDR2 phosphorylated at site Tyrosine-740 as well as total DDR2.

Characteristics:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for DDR2

Target: DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))

Alternative Name: DDR2 (DDR2 Products)

Synonyms: MIG20a ELISA Kit, NTRKR3 ELISA Kit, TKT ELISA Kit, TYRO10 ELISA Kit, AW495251 ELISA Kit, Ntrkr3 ELISA Kit, tyro10 ELISA Kit, DDR2 ELISA Kit, Tyro10 ELISA Kit, ddr2 ELISA Kit, si:ch211-193c2.1 ELISA Kit, discoidin domain receptor tyrosine kinase 2 ELISA Kit, discoidin domain receptor family, member 2 ELISA Kit, discoidin domain receptor family, member 2, like ELISA Kit, DDR2 ELISA Kit, Ddr2 ELISA Kit, ddr2l ELISA Kit

Gene ID: 4921

UniProt: Q16832

Pathways: RTK Signaling