BLK ELISA Kit
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- Target See all BLK ELISA Kits
- BLK (B Lymphoid Tyrosine Kinase (BLK))
- Binding Specificity
- pTyr
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Custom Human Phosphotyrosine BLK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine BLK in lysate samples.
- Sample Type
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- Specificity
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-BLK.
- Characteristics
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Expiry Date
- 6 months
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- Target See all BLK ELISA Kits
- BLK (B Lymphoid Tyrosine Kinase (BLK))
- Alternative Name
- BLK (BLK Products)
- Synonyms
- bltk ELISA Kit, si:dkey-33i22.2 ELISA Kit, zgc:136231 ELISA Kit, MODY11 ELISA Kit, BLK proto-oncogene, Src family tyrosine kinase ELISA Kit, B lymphoid kinase ELISA Kit, blk ELISA Kit, BLK ELISA Kit, Blk ELISA Kit
- Gene ID
- 1057
- UniProt
- P51451
- Pathways
- Positive Regulation of Peptide Hormone Secretion, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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