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DIABLO ELISA Kit
DIABLO
Reactivity: Rat
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details DIABLO ELISA Kit
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Target
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DIABLO
(Second Mitochondria-Derived Activator of Caspase (DIABLO))
Reactivity
All reactivities for DIABLO elisa kits
Rat
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Rat SMAC ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes Rat SMAC.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for DIABLO
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Target
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DIABLO
(Second Mitochondria-Derived Activator of Caspase (DIABLO))
Alternative Name
SMAC (DIABLO Products )
Synonyms
smac ELISA Kit, smac3 ELISA Kit, diablo-s ELISA Kit, 0610041G12Rik ELISA Kit, 1700006L01Rik ELISA Kit, AU040403 ELISA Kit, Smac ELISA Kit, DFNA64 ELISA Kit, SMAC ELISA Kit, DBO ELISA Kit, XSmac ELISA Kit, diablo, IAP-binding mitochondrial protein ELISA Kit, diablo IAP-binding mitochondrial protein ELISA Kit, diablo, IAP-binding mitochondrial protein L homeolog ELISA Kit, diablo ELISA Kit, Diablo ELISA Kit, DIABLO ELISA Kit, diablo.L ELISA Kit
Gene ID
288753
UniProt
Q5RK17
Pathways
Apoptosis , Caspase Cascade in Apoptosis , Positive Regulation of Endopeptidase Activity
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