BIRC3 ELISA Kit

Catalog No. ABIN4885857

Product Details BIRC3 ELISA Kit

Target: BIRC3 (Baculoviral IAP Repeat Containing 3 (BIRC3))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Rat cIAP-2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Rat cIAP-2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for BIRC3

Target: BIRC3 (Baculoviral IAP Repeat Containing 3 (BIRC3))

Alternative Name: cIAP-2 (BIRC3 Products)

Synonyms: AIP1 ELISA Kit, API2 ELISA Kit, CIAP2 ELISA Kit, HAIP1 ELISA Kit, HIAP1 ELISA Kit, MALT2 ELISA Kit, MIHC ELISA Kit, RNF49 ELISA Kit, c-IAP2 ELISA Kit, Api1 ELISA Kit, Api2 ELISA Kit, IAP1 ELISA Kit, IAP2 ELISA Kit, MIHB ELISA Kit, Birc2 ELISA Kit, HIAP2 ELISA Kit, MIAP1 ELISA Kit, MIAP2 ELISA Kit, cIAP1 ELISA Kit, cIAP2 ELISA Kit, cIAP-1 ELISA Kit, cIAP-2 ELISA Kit, AW107670 ELISA Kit, BIRC2 ELISA Kit, baculoviral IAP repeat containing 3 ELISA Kit, baculoviral IAP repeat-containing 3 ELISA Kit, baculoviral IAP repeat-containing protein 3 ELISA Kit, inhibitor of apoptosis protein-2 ELISA Kit, BIRC3 ELISA Kit, Birc3 ELISA Kit, LOC100399118 ELISA Kit, OpmnVgp074 ELISA Kit

UniProt: F7FLN8

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades