HSP27 ELISA Kit

Catalog No. ABIN4885540

Product Details HSP27 ELISA Kit

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Mouse Heat Shock Protein 27 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Mouse Heat Shock Protein 27.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27 (HSPB1 Products)

Synonyms: CMT2F ELISA Kit, HMN2B ELISA Kit, HS.76067 ELISA Kit, HSP27 ELISA Kit, HSP28 ELISA Kit, Hsp25 ELISA Kit, SRP27 ELISA Kit, 27kDa ELISA Kit, Hsp27 ELISA Kit, cb153 ELISA Kit, cb660 ELISA Kit, hsp1 ELISA Kit, hsp25 ELISA Kit, hsp27 ELISA Kit, id:ibd2821 ELISA Kit, sb:cb660 ELISA Kit, zgc:103437 ELISA Kit, HSPB1 ELISA Kit, cmt2f ELISA Kit, hs.76067 ELISA Kit, hsp28 ELISA Kit, heat shock protein family B (small) member 1 ELISA Kit, heat shock protein 1 ELISA Kit, heat shock protein, alpha-crystallin-related, 1 ELISA Kit, heat shock protein family B (small) member 1 L homeolog ELISA Kit, HSPB1 ELISA Kit, Hspb1 ELISA Kit, hspb1 ELISA Kit, hspb1.L ELISA Kit

Gene ID: 15507

UniProt: P14602

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling