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Chemerin ELISA Kit
RARRES2
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details Chemerin ELISA Kit
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Target
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Chemerin (RARRES2)
(Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))
Reactivity
All reactivities for Chemerin elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Mouse Chemerin (RARRES2/TIG2) ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes mouse Chemerin.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for Chemerin
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Target
See all Chemerin (RARRES2) ELISA Kits
Chemerin (RARRES2)
(Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))
Alternative Name
Chemerin (RARRES2 Products )
Synonyms
HP10433 ELISA Kit, TIG2 ELISA Kit, 0610007L05Rik ELISA Kit, AI303516 ELISA Kit, retinoic acid receptor responder 2 ELISA Kit, retinoic acid receptor responder (tazarotene induced) 2 ELISA Kit, RARRES2 ELISA Kit, Rarres2 ELISA Kit
Gene ID
71660
UniProt
Q9DD06
Pathways
Brown Fat Cell Differentiation
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