FGF1 ELISA Kit

Catalog No. ABIN4885416

Product Details FGF1 ELISA Kit

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 12-3000 pg/mL

Minimum Detection Limit: 12 pg/mL

Application: ELISA

Purpose: Mouse aFGF (FGF-1) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Mouse Fibroblast Growth Factor acidic (FGF1)

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for FGF1

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Alternative Name: aFGF (FGF1 Products)

Synonyms: AFGF ELISA Kit, ECGF ELISA Kit, ECGF-beta ELISA Kit, ECGFA ELISA Kit, ECGFB ELISA Kit, FGF-1 ELISA Kit, FGF-alpha ELISA Kit, FGFA ELISA Kit, GLIO703 ELISA Kit, HBGF-1 ELISA Kit, HBGF1 ELISA Kit, Fgf1 ELISA Kit, FGF1 ELISA Kit, aFGF ELISA Kit, ecgf ELISA Kit, fgfa ELISA Kit, ecgfa ELISA Kit, ecgfb ELISA Kit, fgf-1 ELISA Kit, hbgf1 ELISA Kit, glio703 ELISA Kit, ecgf-beta ELISA Kit, fgf-alpha ELISA Kit, Dffrx ELISA Kit, Fam ELISA Kit, Fgf-1 ELISA Kit, Fgfa ELISA Kit, zgc:136885 ELISA Kit, LOC100221393 ELISA Kit, fgf1 ELISA Kit, zgc:73249 ELISA Kit, fibroblast growth factor 1 ELISA Kit, fibroblast growth factor 1 L homeolog ELISA Kit, fibroblast growth factor 1b ELISA Kit, fibroblast growth factor 1a ELISA Kit, FGF1 ELISA Kit, fgf1.L ELISA Kit, fgf1 ELISA Kit, Fgf1 ELISA Kit, fgf1b ELISA Kit, LOC100221393 ELISA Kit, fgf1a ELISA Kit

Gene ID: 14164

UniProt: P61148

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway