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GAS6 ELISA Kit

GAS6 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4885228
  • Target See all GAS6 ELISA Kits
    GAS6 (Growth Arrest-Specific 6 (GAS6))
    Reactivity
    • 6
    • 3
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human Gas 6 SpeedELISA Kit.
    Brand
    SpeedELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Compressed workflow with only one wash step and three incubation steps
    • 3 hours total processing time
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Streptavidin-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Capture Antibody
    • HRP Conjugated Detection Antibody
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Sample Volume
    100 μL
    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 70 μL of the Antibody Cocktail to each well.
    3. Incubate 10 minutes at RT.
    4. Add 50 μL of sample or standard to each well.
    5. Incubate 2 h at RT.
    6. Wash Wells
    7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    8. Incubate 30 min at RT.
    9. Add 50 μL of Stop Solution to each well.
    10. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
    Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    Expiry Date
    6 months
  • Target See all GAS6 ELISA Kits
    GAS6 (Growth Arrest-Specific 6 (GAS6))
    Alternative Name
    Gas 6 (GAS6 Products)
    Synonyms
    fj34a12 ELISA Kit, zgc:63860 ELISA Kit, wu:fj34a12 ELISA Kit, MGC68463 ELISA Kit, gas6 ELISA Kit, MGC83872 ELISA Kit, GAS6 ELISA Kit, Gas-6 ELISA Kit, AXLLG ELISA Kit, AXSF ELISA Kit, growth arrest specific 6 ELISA Kit, growth arrest-specific 6 ELISA Kit, growth arrest specific 6 L homeolog ELISA Kit, growth arrest specific 6 S homeolog ELISA Kit, GAS6 ELISA Kit, gas6 ELISA Kit, gas6.L ELISA Kit, gas6.S ELISA Kit, Gas6 ELISA Kit
    Gene ID
    2621
    UniProt
    Q14393
    Pathways
    RTK Signaling, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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