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WNT4 ELISA Kit
WNT4
Reactivity: Human
Colorimetric
Sandwich ELISA
1.22-300 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details WNT4 ELISA Kit
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Target
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WNT4
(Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))
Reactivity
All reactivities for WNT4 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
1.22-300 ng/mL
Minimum Detection Limit
1.22 ng/mL
Application
ELISA
Purpose
Human Wnt-4 ELISA Kit for serum, plasma, and cell culture supernatant samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
This ELISA antibody pair detects human Wnt-4. Other species not determined.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for WNT4
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Target
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WNT4
(Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))
Alternative Name
Wnt-4 (WNT4 Products )
Synonyms
CG4698 ELISA Kit, DWnt-4 ELISA Kit, DWnt4 ELISA Kit, Dm DWnt4 ELISA Kit, Dmel\\CG4698 ELISA Kit, Dwnt4 ELISA Kit, Wnt ELISA Kit, Wnt-4 ELISA Kit, anon-EST:Liang-2.4 ELISA Kit, clone 2.4 ELISA Kit, wnt-4 ELISA Kit, wnt4 ELISA Kit, SERKAL ELISA Kit, WNT-4 ELISA Kit, Xwnt4 ELISA Kit, xwnt-4 ELISA Kit, zgc:136737 ELISA Kit, Wnt family member 4 ELISA Kit, Wnt oncogene analog 4 ELISA Kit, wingless-type MMTV integration site family, member 4 ELISA Kit, wingless-type MMTV integration site family member 4 S homeolog ELISA Kit, wingless-type MMTV integration site family, member 4a ELISA Kit, WNT4 ELISA Kit, Wnt4 ELISA Kit, wnt4.S ELISA Kit, wnt4a ELISA Kit
Gene ID
54361
UniProt
P56705
Pathways
WNT Signaling , Regulation of Hormone Metabolic Process , Regulation of Hormone Biosynthetic Process , Cell-Cell Junction Organization , Tube Formation
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